Investigating the Effect and the Optimal Seeding Ration of an Autologous Mesenchymal Stem Cell and Chondrocyte Co-culture

Description

This video presentation features Christopher Mak discussing his research project focused on the effects and optical seeding ratio in a co-culture of autologous mesenchymal stem cells and chondrocytes, particularly regarding their application in treating osteochondral lesions caused by osteoarthritis and sports injuries. He addresses the variability of seeding ratios used in previous studies—ranging from 1:1 to as high as 100:1—and identifies the lack of consistency in findings related to these ratios. Through his investigation, which involved seven patients with advanced osteoarthritis, Mak explores how a 1:1 ratio significantly upregulates important cartilage-related proteins compared to higher ratios. He suggests that these results could influence future clinical practices and points out the importance of re-evaluating previous studies that utilized suboptimal ratios. The presentation concludes with a discussion of potential future experiments to further investigate the optimal ratios for effective stem cell therapy in osteoarthritis treatment.

DOI: 10.1302/3114-221044

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