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- Talk
- 13/09/2021
- UK
Modification of Decellularisation Methods Assess the Effects of Swelling on the Mechanical Properties and Composition of Porcine Tendon
Description
This VTT captures a presentation by Jacqueline Solis, a final year PhD student at the University of Leeds, discussing ACL (anterior cruciate ligament) reconstruction. The presentation begins with an acknowledgment of technical issues and smoothly transitions into a detailed overview of the ACL, which is noted as the most frequently injured ligament in the knee. Jacqueline emphasizes the surgical replacement of the ruptured ACL through reconstruction, highlighting its prevalence with over 400,000 procedures performed annually worldwide.
She discusses the use of decellularized porcine superflexor tendon as a graft material that serves as a viable alternative for ACL reconstruction, providing a biocompatible structure that promotes tissue remodeling and stability. The presentation outlines the methodology involved in decellularization, including the role of hydration solutions such as PBS (phosphate-buffered saline) and its effects on the mechanical properties of the tendon.
Jacqueline shares results from her experiments involving alternative solutions for tissue hydration during decellularization and their influence on the swelling and mechanical behavior of the superflexor tendon. She presents findings showing that different solutions resulted in varying degrees of swelling and strength, with 25% Ringer's solution producing the best outcomes in terms of mechanical properties when tested.
The conclusion summarizes that reducing tissue swelling is linked to improved mechanical properties, and while altering the solution during the decellularization process does not impact the quantity of matrix components like GAGs (glycosaminoglycans) and collagen, it affects the structure and function of the decellularized tendon. Jacqueline's findings support the potential of all tested decellularized tendons to serve as suitable grafts for ACL reconstruction. She ends by thanking the audience and inviting further communication through chat for questions.